Place it right after the hit lands in your timeline, then follow a bigger fall with Roblox Oof Sound for a second beat. For a physical gag, lead in with Doge Bonk Sound Effect and let this one answer it as the payoff line.
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A sub-second, high-pitched hurt yelp from Mario, voiced as a sharp rising grunt that ends almost as soon as it starts. It feels cartoonish and over-the-top rather than serious, turning a small mistake into instant comic pain. The clip comes from Super Mario 64, the 1996 Nintendo 64 platform game, where it plays when Mario takes damage.