The clip comes from Super Mario 64, the 1996 Nintendo 64 platform game where Mario makes a distinct hurt noise whenever he takes damage from an enemy or a fall. As gameplay footage, speedruns, and Mario memes spread across YouTube and social media, the short yelp became a handy shorthand for comic pain that needed no explanation. Soundboards and short-form editors kept it alive long after the game itself, because a single second of that familiar voice instantly tells viewers a character has messed up. Its brevity and nostalgia keep it useful in new edits today.