Place it at the start of a fail sequence, just before the mistake lands, then cut to the Nope Meme Sound, and save the No God Please No clip for the bigger disaster that follows.
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A two-second spoken clip repeats “oh no” again and again, the voice climbing higher with each repeat. It lands as sudden dread, like catching a mistake at the exact point it can no longer be undone. The clip comes from Uncharted.