Place it right after the failed attempt is shown, then follow it with Sad Violin for a send-off, or Wrong Answer Game Show when the mistake was obvious. Keep it as the reaction beat, not the opening, so the loss lands first.
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This is a short spoken clip of the Portuguese phrase 'Se foi o boi com a corda', lasting about two seconds. It carries a resigned, comic feeling for a moment when something has slipped away and cannot be pulled back, like admitting defeat before anyone else can point it out.