Put it near the start of a reaction beat, then follow it with Pop Sound Effect for a second tiny hit before the main joke lands. For a second step in the chain, add Doge Bonk Sound Effect after it to move from a small surprise to a bigger hit.
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This 0.13-second clip is a single sharp, high squeak that rises quickly and stops almost at once. It feels playful and suddenly silly, making a small moment feel caught off guard without any words. Linked to Undertale, it suits a startled reaction or a tiny character appearing.