Put it on the exact frame a character spots trouble or takes a hit, then follow it with the FNAF jumpscare scream for the reaction and cut to enemy spotted right before the next play starts.
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The Soldier and the Heavy from Team Fortress 2 scream together in this clip, which lasts about two seconds. Their overlapping voices create frantic, over-the-top panic that feels funny and chaotic rather than scary, perfect for exaggerated reactions to sudden in-game failure or surprise.