Place it at the exact moment the bad choice happens in your edit, before any commentary starts. Follow it with a fail sound if the plan collapses, or lead into it after a long pause card to set up the payoff.
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This is a 2.2-second flute cue, a bright little whistle phrase played on a small flute with no voice or backing music. It feels cheeky and mischievous, like a playful warning that someone is about to be taught a lesson. It comes from Willy Wonka & the Chocolate Factory, where Wonka uses the flute to summon the Oompa Loompas.