Place it at the reveal in a stealth-game edit, just before the chase begins, then follow it with Enemy Spotted for the alert and FNAF Jumpscare Scream for the payoff, keeping the knife moment as the turning point of the scene.
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This is a short knife sound effect called Yan Sim Knife 1, lasting about four and a half seconds and centred on a single sharp knife effect. It gives a tense, uneasy feeling, like a quiet moment suddenly turning dangerous, and it works best as a sudden warning rather than background noise.