Place it at the end of a short reaction edit, after a sad meow and before a nya cat girl clip. That order gives the insult a clear target, then lets the next cat sound follow it with a reply for viewers who enjoy animal exchanges.
このサウンドについて
This short clip turns a cat's meow into the Italian insult coglione, delivered in about five seconds with a blunt, deadpan edge that makes the insult land harder. It feels rude, funny and unexpectedly smug, like a pet passing judgment on a bad idea. The effect comes from hearing a familiar animal sound carry a very human insult.