Place it right after the reaction beat in your edit, then follow it with the Re:Zero Return By Death sound to sell the loop, or precede it with the Anime Ahh sound for a stacked anime reaction sequence.
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This clip is a short 1.7-second high-pitched scream of EIEEEEH, a drawn-out shriek delivered in a panicked anime style. It feels frantic and funny, like an exaggerated overreaction that turns shock into a joke. It comes from Gigguk's Re:Zero IN 8 MINUTES recap.