Place it just before the reveal in a gaming montage, after a tense build-up, then follow it with a jumpscare scream for the payoff. For a slower villain entrance, open with a danger alarm and let this clip close the moment.
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This is the short, just-under-five-second maniacal clown laugh from Twisted Metal, a gleeful burst of cackling that sounds delighted by mayhem. It feels sinister and playful at once, like a villain enjoying the chaos a little too much, and it works as an instant signal that trouble has arrived.