Put it at the peak of a scare, after a short suspense cue and before the reaction shot, then follow it with a Vine Boom to turn the fright into a joke. For a failed prank, place a SpongeBob Fail after it instead, letting the second sound confirm the scare did not work.
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This is a roughly 12-second drawn-out ahhhh scream, held long enough to sound like someone reacting in real panic rather than giving a quick yelp. It feels startling and theatrical at once, the kind of over-the-top shriek that makes a jump scare land harder and turns a sudden fright into something funny.