This clip appears to be a user-generated soundboard upload rather than a line or effect taken from a known film, show or game, with its file metadata simply labelling it as a mother fart joke. That anonymity is part of why it circulates so easily, as creators can drop it into any prank, meme or reaction video without needing context or recognition. Fart sounds have long been a staple of slapstick and internet humour, and short, cartoonish versions like this one remain popular because they are instantly understood, easy to time and get a laugh from a broad audience.