Place this clip at the very end of a Halloween montage, after the last visual gag, so it closes the edit as the final beat. Build tension first with Metal Gear Alert, then cut straight to this scream, and finish with Law and Order Dun Dun as a deadpan punchline once the scare has landed.
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This is a brief, piercing scream lasting about three seconds, with a sudden rise that cuts through everything around it and then stops dead. It feels startling and darkly funny, like a jump scare landing in the middle of a cartoon, and it works best when the shock is the joke. It comes from the Gracie Films closing logo as heard in a Simpsons Treehouse of Horror episode.