Place this clip at the end of a fail sequence as the final reaction, after a build-up sound. Pair it after the SpongeBob Boowomp Sound Effect for a softer letdown first, or follow it with the Womp Womp Fail Sound when you want a second, sadder drop.
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This clip is Nelson Muntz's short 'Ha ha!' taunt from The Simpsons, a pointed two-syllable laugh delivered in under two seconds. It feels gleefully mean and instantly mocking, like a playground jeer aimed at someone who just slipped up. The laugh comes from the long-running animated series where Nelson uses it to ridicule other characters' mistakes.