Place this clip at the climax of a scare sequence, after an it was at this moment he knew setup and before a fail sound meme payoff, so the panic becomes the centerpiece before the joke lands and the scene can move on.
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This clip is Marv's extended yell from the spider scene in Home Alone, a single panicked scream that keeps going for almost fifteen seconds. It feels frantic, overblown and funny, turning a small scare into total chaos and making the listener share the shock before laughing at how long the panic lasts.