Place it at the exact frame the chest opens, after a short warning cue and before the reaction that follows the ambush, pairing it first with Danger Alarm Meme Sound and then with No God Please No for the aftermath.
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The clip is the short death cry of a Mimic, a chest-shaped monster that attacks anyone who tries to open it, lasting just under three seconds as a raspy screech turns into a strained, human-like wail. It feels funny and startling at once, like a betrayal that hurts the betrayer too, and it comes from Dark Souls, the 2011 action role-playing game by FromSoftware.