Place it at the end of a prank or villain reveal, after Sudden Suspense builds the tension and just before the payoff lands. Follow it with Nelson's Ha-Ha! for a second beat, or swap in Laughing Dog Meme when the joke needs a sillier animal reaction instead.
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This short clip is a high-pitched, raspy gremlin laugh that bursts out in fast, wheezy cackles for just over three seconds. It sounds manic and gleefully unhinged, like a tiny villain who has just pulled off a prank and cannot stop giggling. The effect is instantly silly and a little creepy, perfect for a moment that is funny because it feels completely over the top.