Place it at the very opening of a morning or farm clip, before any dialogue or music begins. Follow it with the Bell Ding Sound Effect Button for a tidy wake-up cue, or cut to the Dun Dun Dun Sound Button when the early start turns into a joke.
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This short clip is a loud rooster crow lasting about two and a half seconds, with a sharp rising call that sounds like a farmyard alarm going off at dawn. It feels abrupt and comic, part wake-up call and part prank, jolting listeners awake before the humour lands.