Place this clip at the reveal in your edit, right after the animal appears, then follow it with the Vine Boom Sound Button for a second punch. It also works before a Classic Fart Sound Button when cutting to a sillier animal clip.
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This is a brief 1.3-second recording of a sea lion letting out a loud, honking bark. The sudden animal call feels absurd and startling, turning an ordinary moment into a confused, what-is-it-doing reaction that works because the noise is so unexpected and unmistakably not human.