Put it immediately after the mistake lands, then follow it with Oh No No No Laugh to stretch the reaction. For a bigger build-up, open with Shocked Meme Sound Effect before the error, then let this clip deliver the verdict.
Sobre este som
This is a roughly three-second shout of Seu Madruga yelling "Burro!" in a sharp, annoyed voice from El Chavo del Ocho. The delivery feels like a comic scolding, funny and pointed rather than angry, and it lands best as a quick jab at an obvious blunder. It comes from the Mexican television series, where Ramón Valdés played the character.