Place it just after the snake is revealed, following It Was At This Moment He Knew to frame the danger, and follow it with Fail Sound Meme once the reaction has landed to close the joke.
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This short voice clip features Indiana Jones recoiling at the sight of snakes, asking in weary disbelief why they had to be the danger. It lasts just under five seconds and captures his reluctant dread in a single exasperated line. The moment is funny because his fear feels so human, turning a heroic adventurer squeamish. It comes from Raiders of the Lost Ark, where his phobia of snakes is revealed.