Place it at the reveal moment in a twin comparison edit, after the Law and Order Dun Dun sting sets up the discovery and before a You What reaction closes the sequence for confused friends.
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A short two-second voice clip of Stewie Griffin delivering a dry line about his twin brother in his posh, plotting tone. It feels cheeky and oddly smug, turning a family reveal into a quick deadpan laugh. The line comes from the animated television world of Family Guy, where his schemes drive the joke.