Place Dun Dun Dun just before this clip in a night scene, then let this clip carry the reveal, and follow it with a low, distant rumble to close the scene.
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This clip is a single, drawn-out howl from a she-wolf, lasting about seven seconds, rising and falling like a lone animal calling across the night. It feels wild, eerie and lonely, with a raw edge that makes a quiet scene feel suddenly exposed and tense.