Place this clip at the reveal point of the edit, after a dun dun dun buildup and before a vine boom for the aftermath. That order gives the scare a clear setup and a heavy punctuation mark without needing extra effects layered underneath.
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This is a 2.5-second clip of a female scream recorded as a horror sound effect, high-pitched and abrupt from a lone voice. It feels tense and startling, turning an ordinary moment into a sudden scare, and the short hit makes the shock land instantly rather than building slowly over time.