Put this right after the mistake visual in your timeline, before you cut to the reaction face. Follow it with the Vine Boom Sound Button for an oversized fail, or swap it for the SpongeBob Fail Sound Button when you want a sadder closer instead.
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This is a one-second voice clip of someone blurting out a sharp, whiny 'owgh' like they just stubbed their toe. It feels pained and a little ridiculous, so the sting reads as funny rather than serious and the short hit makes it easy to drop on a slip-up.