Place it right on the odd frame, then follow it with Goofy Ahh Sounds to keep the joke going before the scene moves on. For a deadpan reaction to the same frame, use Bruh Sound Effect after the cut instead, so the two jokes do not compete in one moment.
About this sound
This is a short, offbeat sound effect, lasting about nine seconds, with an abrupt, goofy burst that lands like a sudden jolt. It feels awkward, confusing and unexpectedly funny, turning a normal cartoon frame into something cursed and strange. Creators reach for it when a face, transition or zoom looks wrong, because the noise makes the odd moment feel even more absurd.