Place it right at the reveal in your edit, then follow it with a bruh soundboard button for the deadpan drop-off, or stack an oh my god meme sound button before it to build the reaction in two steps.
About this sound
This is a very short voice clip of someone shouting the Portuguese phrase 'Ah essa velha Madonna!' in one quick burst lasting about 1.3 seconds. The delivery feels teasing and exasperated, like a playful call-out, which makes the line land as funny and a little dramatic even if you only catch the tone.