Place it at the peak of a chase or reveal, after a slow build-up, then follow it with a run cue to keep the escape moving. For a bigger reaction, put the shocked exclamation before it, so the scene builds from disbelief to full flight.
About this sound
A frightened voice warns that someone is coming, then breaks into a loud, high-pitched scream that lasts just over two seconds. The sudden jump from a clear warning to full panic feels frantic and funny, especially when the threat turns out to be small or harmless. It works as a quick burst of chaos for chase scenes, failed plans and exaggerated reactions.