Place it at the very end of a reaction sequence as the final reply, after the bruh sound effect has marked the fail. For a bigger pile-on, follow it with emotional damage, then stop and let the scene move on.
About this sound
A 7-second Portuguese voice clip of a man saying “aí cê me quebra, né amigo” in a casual, exasperated tone. It gives off teasing disbelief and mild annoyance, like hearing a terrible excuse or an awkward comment from a friend, playful rather than genuinely angry.