Place it just before the reveal shot, after the suspect has been named, so the accusation has room to land. Follow it with a dramatic dun dun dun for a second beat, then a vine boom when the guilty face is finally shown to close the joke.
About this sound
This 4.6-second clip is a rising synth hum that builds before breaking into a metallic ping, the cue that tells an Among Us player whether they are a Crewmate or an Impostor. It feels tense and watchful, like a secret is about to slip out. It comes from InnerSloth's 2018 game Among Us, where it plays at the start of each match to reveal a player's hidden role.