Place it at the very end of a reveal sequence, after the setup clip has finished, so it closes the joke. For a bigger escalation, follow it with an Oh My God reaction, or open with Emotional Damage before the reveal for a contrast that makes the payoff hit harder.
About this sound
This clip holds one long, theatrical wow in a bright anime-style voice, with light comic music playing underneath, for about four seconds. It starts as startled disbelief and swells into pure silliness, giving listeners an overblown, laugh-out-loud payoff that turns an ordinary reveal into a joke in itself. The fun is in how much bigger the reaction feels than the moment deserves.