In a gaming edit, put this clip after the failed play itself, following an Enemy Spotted cue for the threat and finishing with a Nope reaction if the player tries again. That order builds the setup, pays it off, then closes the joke.
About this sound
This clip is a short laugh lasting about 1.3 seconds, the English Taunt 11 known as Herb Laugh. It gives a listener an amused, teasing feeling, like someone cracking up at a rival's mistake right in front of them. It comes from Age of Empires II.