Place it at the end of a failed attempt, then follow it with the SpongeBob fail cue to underline the flop, or let the classic error tone close the scene after the question hangs in the air.
About this sound
This 8-second clip pairs a Spanish voice asking whether the car will start with the sound of an engine cranking again and again without catching. It feels teasing and exasperated, like watching a stubborn car refuse to cooperate on a cold morning. It comes from a Hescher spark plugs advertisement, which uses the failed start to sell the fix.