Place it at the end of a fail sequence as the final reaction, then follow it with the damn meme sound for a second hit, or precede it with a faaaa meme sound to build the sequence before the closing beat.
About this sound
A drawn-out male voice stretches out 'awwwww man' in one long, drooping delivery that lasts just under two seconds. The clip captures pure disappointment, sounding deflated, resigned and a little dramatic, which makes even a small setback feel funny and overblown to anyone listening nearby.