Place it at the start of a scene showing unanswered messages, then cut to the empty room. Follow it with the Bruh sound when nobody answers again, and save the Emotional Damage sound for the final reaction when nobody ever replies.
About this sound
This is a short Spanish voice clip, just over two seconds long, of someone asking '¿Hay alguien aquí con vida?' — is there anyone here alive? The question makes it feel funny and awkward, like calling out into an empty room and waiting for a reply that never comes.