Place it at the contact point in a blooper or gameplay edit, after the visual setup and before the reaction line. Follow it with the Mario Jump Sound Button for a second hop, or precede it with a Dun Dun Dun cue for a bigger reveal.
About this sound
A 0.39-second cartoon boink effect pitched in G major, a single springy bounce hit with a bright, elastic tone. It gives a listener a light, goofy lift, turning a small bump or jump into a playful, silly beat rather than a serious impact.