This sound is best understood as a generic booing reaction rather than a clip with a clearly verified single origin. Short boo sounds have long been used in comedy, sports, live TV, and internet edits to signal instant disapproval. Online, creators keep using them because the meaning is obvious in less than a second, which makes them ideal for punchy meme timing. It fills a similar reaction role to a simple nope, turning a weak joke, awkward post, or failed move into an instantly readable gag.