This clip is best understood as a generic cartoon impact effect rather than a quote from one famous scene. Sounds like this became popular through slapstick animation language and later spread widely in meme edits, short videos and gaming montages. Creators keep using the cartoon hammer hit because it communicates a joke immediately, even in a very short clip. Online, it often appears alongside other comedic reaction sounds and exaggerated bonk edits to make small visual moments feel much bigger.