Place it right after the mishap in your edit, then follow it with SpongeBob Boowomp to underline the failure, or with Womp Womp Fail for a second beat on the same joke.
About this sound
This short clip captures Eustace, the grumpy farmer from Courage the Cowardly Dog, letting out a loud, drawn-out 'OWWW' after hurting himself. Lasting just over a second, the single pained yell feels sudden, silly and sharply comic, like a cartoon pratfall landing in one beat. It comes from the animated series, where the farmer often suffers for his bad temper.