Place this clip right after the bad decision lands, before the reaction shot cuts away. Build tension first with a sudden suspense sting, then follow this shout with a no no wait wait button if the character tries to backtrack and fails.
About this sound
The clip is Cowboy Dave shouting 'No, no, no, no, NO' for about five seconds as trouble closes in, his voice rising from quick refusals to a full panicked yell. It feels frantic and funny at once, like pure terrified disbelief. It comes from Kids A-Z, where Dave and his horse Shorty flee into the desert.