Place it at the start of the sprint in your edit, after a warning cue and before the running clip, so the sequence reads as stop, go, then chase. Open with Enemy Spotted, follow with this clip, then cut to Run Vine as players scatter, and close with Okay Let's Go for the next round.
About this sound
This is the half-second 'Green' call from Crab Game's Red Light, Green Light round, a single clipped voice cue that tells players to run. It lands with a sudden rush of tension and relief, the exact beat when frozen players burst forward again. It comes from Crab Game, the multiplayer party game where players race on that call.