Put it right at the reveal in a gaming clip, after a short build-up from the Inception Horn, then let the FNAF Jumpscare Scream follow as the second scare in the sequence.
About this sound
This is a short 1.75-second clip of Crash Bandicoot letting out a rising, stretched-out woah that suddenly cuts in like a jumpscare. It feels startling and silly at the same time, the kind of abrupt cartoon panic that makes people flinch and then laugh. It comes from the games, where the bandicoot's exaggerated woah is part of his character.