Place it at the end of a failed play or bad suggestion, then follow it with a shocked reaction sound to sell the disbelief. For a chase or escape fail, cut to the why are you running meme next, letting the refusal set up the bigger payoff that follows.
About this sound
This under-two-second voice line from Counter-Strike: Global Offensive features an Elite Crew character calmly saying 'No, my friend' in a dry, dismissive tone. It feels playfully sarcastic rather than angry, turning a simple refusal into a knowing correction. Its clean delivery makes failed plans and confident mistakes land as light mockery.