Place it at the end of a cursed edit, after a goofy ahh sound sets up the absurdity, then follow it with an emotional damage hit to underline the bad decision. Keep the sequence short so the contrast stays the punchline.
About this sound
This roughly ten-second clip puts a cheerful SpongeBob image against a long, exaggerated Ambatakam vocal that keeps rising until it feels over the top. The mismatch makes it awkward and funny in an uncomfortable way, like a normal cartoon moment tipping into something unhinged. It works best when the reaction needs to feel deliberately wrong rather than just loud.