Put it at the peak of a reaction shot, just after the character notices the challenge, then follow it with Anime Ahh for a flustered retreat and a Boing Boing for the bounce back into place.
About this sound
This is a brief, roughly four-second playful roar with a stylised anime energy, pitched more cute than threatening and closer to a tiny battle cry than a monster growl. It feels funny, mock-fierce and deliberately over-the-top, giving a pet, character or creator a burst of exaggerated attitude that stays adorable rather than harsh. The contrast keeps the listener smiling while the roar still lands as a joke.