Place it at the end of a failed chase or botched escape, then follow it with the Mission Failed sting to confirm the defeat, or open with the Drama sting before the laugh to build the reveal. For a game-over beat, add the GTA V Wasted cue after the laugh instead of another joke sound.
About this sound
This is a roughly three-second burst of The Doctor's laugh from Dead by Daylight, a sharp, manic cackle that rises and breaks with unsettling glee. It feels threatening and darkly funny at once, turning a small failure or reveal into something menacing. The clip comes from Behaviour Interactive's asymmetrical horror game, where The Doctor is one of its killer characters.