Place it at the peak of a visual gag, then follow it with a game show buzzer for a clear fail or a goofy ahh sounds cue when the moment needs a second punch, keeping the boing as the first beat in the sequence.
About this sound
This sub-second clip is a quick, springy boing that rises fast and drops back with a wobbly finish, like a rubber band flicked in a cartoon. It feels light, silly and deliberately over the top, turning a small slip or surprise into a joke instead of a mishap. Its brevity makes it work as a sharp comic accent rather than a longer effect.