Place this clip as the final beat after the question itself, not under background chatter. Follow it with a nope sound for an instant shutdown, or precede it with the FBI Open Up button when the interrogation joke needs building first.
About this sound
This is Mrs. Puff delivering her full warning line in a flat, teacher-like voice, lasting about nine seconds as she tells the listener not to ask questions they cannot handle the answer to. It feels dryly funny and a little ominous, like a scolding that is also a dare. The clip comes from SpongeBob: Battle for Bikini Bottom.