Put it at the end of a rising tension beat, after Dun Dun Dun and just before the reveal lands. Follow it with Vine Boom for a comic reset, letting the second hit turn the moment into a joke instead of leaving viewers hanging.
About this sound
This is a very short, under-a-second scream built for a jumpscare, sharp and sudden with no build-up. It lands with a jolt of surprise and nervous laughter, the kind of scare that makes people flinch before they laugh. It comes from a Five Nights at Freddy's style duck jumpscare moment, which is why it works best as a quick shock.